|Methyltransferase Activity of the EZH1 Complex.
Methylation determined as TCA-precipitable counts in in a scintillation/filter plate assay. Reactions were 60 min., 30°C, 1 μM [3H]-SAM with 5 μM histone H3 or 0.05 mg/mL core histones.
||Time courses of G9a methyltransferase reactions in the HotSpotSM assay format.|
G9a, at the indicated concentrations, was assayed with 0.05 mg/mL chicken core histones and 1 μM [3H]-SAM. Signal/Background ranged from 4.3 (12.5 nM G9a, 15 min.) to 51.2 (50 nM G9a, 60 min.).