AnaBios Corporation

 
AnaBios Corporation社は、ヒトの細胞や組織サンプルを用いて、電気生理やイメージング等のサービスを行っています。痛み、痒み、神経変性疾患、心毒性等、動物実験の結果からヒトでの予測が難しい領域において、Human DRG、Human Heartを用いたPhase-X®の試験が可能です。
DRG, heart tissue, CSF等のヒトサンプルのご購入については、"Purchase Support"ページをご覧ください。

《サービス概要》
◆ Human DRG neurons : Human DRG neuronsを用いたCaイメージングや電気生理、増殖アッセイ等が可能です。
 
イメージング解析
  • Ligand-gated channels and receptors
  • Cold-, warm-, mechano-activated channels and receptors
  • Voltage gated channels
    (proprietary Electrical Field Stimulation technology)
  • In vitro human models for inflammatory pain or neuropathic pain
  • Pruritogen-induced responses (histamine, serotonin, chloroquine etc.) in the presence or absence of pro-inflammatory agents
 
カルシウムイメージングアッセイ
Calcium imaging-based profiling of human sensory neurons responses to chemical and physical stimulations. This technology provides an efficient way to test the activity of new drug candidates on large populations of human neurons.
 
電場刺激によるvoltage gated チャネルの活性化
Electrical Field Stimulation (EFS) of human sensory neurons in culture results in activation of voltage gated ion channels. Following stimulation, the activation of the volatge gated sodium channels is monitored by fluorescence-based imaging of ion influx in the neuron.
 
The pharmachology of EFS-induced responses in human sensory neurons in culture.
電気生理アッセイ 
  • Voltage clamp (voltage-, ligand-gated channels)
    • IC50 determination; frequency-, voltage-dependence
  • Current clamp
    • Modulation of neuronal excitability (step or ramp current injection)
    • Modulation of response to sensitizing agents
    • Modulation of response to pruritogens and sensitizing agents
    • In vitro human models for inflammatory pain or neuropathic pain
 
Voltage clamp-based recordings of voltage gated sodium channels current in human sensory neurons. This methodology allows the profiling of voltage gated channels expressed in human sensory neurons and the determiknation of the potency of novel drug candidates.

Current clamp-based recordings of action potentials in human sensory neurons. The effect of novel drug candidates on human sensory neuron excitability can be assessed in these kind of experiments.

細胞毒性・細胞増殖アッセイ
 
  • Cell viability
  • Apoptosis/necrosis
  • Neurite extension/degeneration
  • ◆ Viable human heart : Viable human heart tissueを用いた電気生理の試験が可能です。
     
    心毒性/安全性試験
    • Drug-induced arrhythmia: sharp electrode recording of membrane potential for assessment of:
      • EAD
      • APD30, APD60 and APD90
      • Short Term Variability
      • Triangulation
    • Drug-induced positive/negative inotropic effects: contraction/relaxation in ventricular trabeculae
    • Drug-induced hyper-/hypo-tension: contraction/relaxation in coronary artery rings
    • Drug-induced valvulopathy
    • Myocardial cytotoxicity
    心疾患研究
    • Electrophysiology-based drug profiling (Atrial fibrillation, Ventricular arrhythmia)
      • Sharp electrode recording of membrane potential
      • Ventricular and atrial tissue
    • Myocardial tissue contractility (heart failure, ventricular hypertrophy)
    • Cardiac mesenchymal stem cells (heart failure, ventricular hypertrophy)
    • Coronary artery disease
    • Valvular disease
      《参考文献》
     
    1. Lee HS, Ghetti A, Pinto-Duarte A, Wang X, Dziewczapolski G, Galimi F, Huitron-Resendiz S, Piña-Crespo JC, Roberts AJ, Verma IM, Sejnowski TJ, Heinemann SF. Astrocytes contribute to gamma oscillations and recognition memory. (2014 ) Proc Natl Acad Sci U S A.; 111 (32): E3343-522)
    2. Davidson S, Copits BA, Zhang J, Page G, Ghetti A, Gereau RW 4th. Human sensory neurons: Membrane properties and sensitization by inflammatory mediators. (2014) Pain; S0304-3959(14)00306-6. doi: 10.1016/j.pain.2014.06.0173)
    3. Arneric SP, Zhang J, Miller P, Rankovic R , Hahn PJ, Gernert DL, Ghetti A Action of biased-mor agonists to inhibit depolarization-induced calcium mobilization in cultured human DRG neurons. (2014) Abstract IASP Meeting
    4. Zhang J, Page P, Enright H, Mcnerney W, Mukerjee E, Wheeler E, Pannu S, Kulp K, Qian F, Miller P and Ghetti A Gadolinium-induced activation of nociceptors in human DRG neuronal-glial cultures. (2014) Abstract IASP Meeting
    5. Arneric SP, Laird JM, Chappell AS, Kennedy JD. Tailoring chronic pain treatments for the elderly: are we prepared for the challenge? (2014) Drug Discov Today; 19: 8-17.
    6. Reynolds J, Ghetti A, Miller P (2013) A robust and reliable ex vivo method for assessing cardiac repolarization using “living” human hearts J Pharmacol Toxicol Method; 68: e167)
    7. Davidson S, Golden JP, Page G, Zhang J, Ghetti A, and Gereau RW Sensory neuron hyperexcitability is prevented by group 2 metabotropic glutamate receptors in mouse and human metabotropic glutamate receptors in mouse and human (2013) Poster at NIH Pain Meeting.
    8. Tate SN, Rugiero F, Derjean D, Panchenko VA, Owen D, Ghetti A, Bannister K, Thakur M, Dickenson AH, Miller P, Morisset V Carbamazepine and CNV1427400 exhibit similar pharmacology in recombinant systems, rat and human native DRGs. (2012) Poster at Society for Neurosciensce Meeting.
    9. Payne L, Panchenko VA, Ghetti A, Alexandrou A, Miller PE, Stevens E Biophysical and pharmacological characterisation of native human Nav1.8 channels from isolated dorsal root ganglia (DRG) (2012) Poster at Biophysical Society Meeting.
    10. Alexandrou A, Turner J, Payne E, Cox PJ, Panchenko VA, Ghetti A, Prime R, Doyle R, Chapman M, Maron B, Miller, PE, Butt R, Stevens E Nav1.7 ios the dominant subtype underlying TTX-s currents in small diameter mouse & human DRGs (2012) Society for Neuroscience Meeting.

    ※掲載されている製品及び受託サービスは全て研究用です。目的を問わず人体への投与を含む、医薬品、診断用医薬品、食品、食品用検査、化粧品等の用途には使用できません。研究用以外の目的には使用しないでください。