Invivotek
Invivotek社は、Genesis Drug Discovery & Development company (GD3)のメンバーで、癌をはじめ、免疫疾患/炎症や代謝疾患等のin vivoモデルを用いて、新薬候補物質の評価を行います。

《サービス一覧》
◆ Oncology & Immuno-Oncology
 
  • Xenograft (CDX) and Syngeneic Tumor models を用いたEfficacy study (実験例)
    •
    モデルのタイプ
    Orthotopic
    Metastatic
    Disseminated
    Subcutaneous    		 投薬方法
    Oral Gavage
    Bolus Injections:
    subcutaneous, Intra-tumoral, Intra-peritoneal, Intravenous, Intra-cardiac, Intramuscular, Intra-articular, Intra-dermal, Intra-ttracheal
    Osmotic Mini-pumps
    In-Diet Administration
  • Imagingによる腫瘍(自然転移・実験的転移等)の観察
    • 同所性及び転移癌の増殖を、in vivoでリアルタイムに観察できます。(実験例)
  • FACS及びマルチプレックス解析
    血液や各種器官の組織からsingle cell suspensionsを作製し、FACS解析を実施。
    腫瘍浸潤リンパ球(Tumor-infiltrating lymphocytes)の同定や、サイトカイン及びホルモン等のマルチプレックス解析の経験も豊富です。
  • Radiotherapy
    • Small Animal Radiation Research Platform (SARRP)を用いた放射線治療も可能です。放射線治療と投薬治療を組み合わせた実験も対応します。(実験例)


    CDX及びSyngeneicモデルの詳細につきましては、お問合せください。
    ◆ Immunology & Inflammation
      免疫及び炎症に焦点を当てた幅広い疾患モデルを用いて、各種アッセイが可能です。
    Animal Disease Models (免疫/炎症疾患)
  • Antigen-induced Rheumatoid Arthritis
  • Collagen-induced Rheumatoid Arthritis
  • Monoclonal antibody induced Rheumatoid Arthritis
  • Experimental Autoimmune Encephalitis (AEA)for Multiple Sclerosis (MS)
  • Allergen-induced pulmonary inflammation for asthma
  • Lipopolysaccharide (LPS)-induced airway inflammation
  • Acute lung injury model (LPS-induced)
  • Dextran Sulfate Sodium (DSS)-induced colitis for ulcerative colitis
  • Trinitrobenzene sulfonic acid (TNBS)- induced colitis for Crohn’s disease
  • Allergic Dermatitis
    [fluorescein isothicyanate (FITC), dinitrofluorobenzene (DNFB) or Oxazolone-induced]
  • Antigen-induced Myasthenia gravis
  • Graft vs. Host Disease

    • Ex Vivo Bioassays
  • Serum and tissue cytokine profiling in animal disease models
  • FACS analysis of lymphoid organs and tissue infiltrates
  • Hematology five-part differential cell count
  • Proliferative responses of T and B cells in vitro
  • In vitro cytokine production assays
  • T cell differentiation assay
    •   ◎ FACSパネル (Multi-Color Panels for FACS Staining)
    ・ Treg Panel・ Inflammatory Infiltrate Panels
    ・ MDSC Panel・ M1/M2 Monocyte/ Macrophage Panel
    ・ Lymphocyte Panel・ White Blood Cell Profiling Panel
    (T and B cells, monocytes, NK cells)
       ◎ 検証済み組織等 (Validated protocols are developed)
    ・ Blood・ Brain and spinal cord
    ・ Spleen・ Liver
    ・ Bone Marrow・ Adipose Tissue
    ・ Lymph Nodes・ Intestine
    ・ Tumor・ Skin
    Cell Therapy Evaluation  セルベースの治療アッセイにも豊富な経験があります。
  • Pluripotent Stem cell therapies for multiple indications in:
      ・ Oncology
      ・ Neuroinflammation
      ・ Autoimmune disorders
  • CAR-T cells (Chimeric Antigen Receptor modified T cells)

    • Non-Disease In Vivo Bioassays
  • Non-lethal endotoxemia with LPS
  • Antigen-dependent in vivo T cell proliferation by BrdU uptake
  • OVA-stimulated in vivo antibody production (IgG1, IgG2a, IgG2b)
  • KLH-stimulated in vivo antibody production (IgM, IgG)
  • Delayed-type hypersensitivity induced by methylated bovine serum albumin (mBSA) or Keyhole limpet hemocyanin (KLH) with adjuvant
  • Delayed-type hypersensitivity induced by SRBC
  • Monocyte or neutrophil infiltration induced by thioglycollate, zymosan, curdlan, or trypsin
  • Cutaneous wound healing
    		•
    ◆ Metabolic Disease Models
      正常なマウスの他、非アルコール性脂肪性肝炎(NASH)や非アルコール性脂肪肝(NAFL)等の疾患モデルを用いた各種アッセイが可能です。
    Animal Disease Models (代謝疾患)
  • Diet-Induced obesity
  • Streptozotocin (STZ)-induced diabetes
  • Genetic models of diabetes and obesity
  • Nonalcoholic steatohepatitis
      ・ Choline-deficient, amino acid defined High Fat Diet (CDA-HFD)
      ・ Neonatal streptozotocin HFD model
      ・ High fat (palm oil), high cholesterol, high fructose obesity model
  • Genetic models of atherosclerosis and dislipidemia

    • In vivo Energy Homeostasis and Body Composition Assessment
  • Longitudinal body weight gain
  • Body composition by DEXA:
      ・ Fat mass/ % fat/ lean mass/ % lean/ total tissue mass/ tissue area
      ・ Bone mineral density and content/ bone area
  • Assessment of regional adipose depots
  • Food intake
  • Indirect calorimetry and locomotor activity:
      ・ O2 consumption/ CO2 production/ respiratory exchange ratio (RER)
      ・ Total horizontal / ambulatory/ non-ambulatory/ vertical activity

    • Glucose Homeostasis
  • Glucose and insulin levels in glucose tolerance tests
  • Gastric emptying
  • Insulin tolerance test
  • Tissue glycogen levels
  • Pancreatic insulin content
  • Glucagon, adiponectin, GLP-1and other circulating hormones

    • Lipid Metabolism
  • Triglyceride levels in oral lipid tolerance tests
  • De novo lipogenesis in vivo
  • Hepatic triglyceride export
  • Serum leptin levels
  • Serum ketone levels
  • Serum and hepatic triglyceride levels
  • Serum free fatty acids
  • Serum and hepatic cholesterol levels
  • Cholesterol distribution: VLDL, LDL, HDL
  • Serum corticosterone levels

    • Cardiovascular Function
  • Non-invasive blood pressure & heart rate with and without L-NMMA Challenge
  • Exercise capacity
  • Histological assessment of atherosclerosis

    • Arteriogenesis
  • Hind-limb ischemia (実験例)
      ・ Includes laser Doppler measured cutaneous blood flow
      ・ Functional clinical score
      ・ Immunohistochemical analysis

    		•
    ◆ Neuroscience
      行動、痛み、運動機能等の様々なアッセイにより、神経疾患に対する候補物質の有効性や毒性を評価します。
  • Neurology
      ・ Locomotor activity (open field, long-term locomotor activity in home cage)
      ・ Rotorod
      ・ Grip strength
      ・ Behavioral Battery
  • Pain
      ・ Acute nociception
      　  - Hot plate
       　 - Acetic acid
       　 - Tail flick
       　 - Hargreaves
      ・ Chronic pain models
       　 - Inflammatory neuritis-carrageenan induced sciatic nerve in rats
       　 - Inflammatory pain–carrageenan and complete Freund’s adjuvant foot pad injection
  • Opioid withdrawal model
  • Affective Disorders
      ・ Elevated Plus maze
      ・ Open field
      ・ Novel environment induced feeding suppression
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