Neurofit SAS
Neurofit SAS社は、CNS(中枢神経系)およびPNS(末梢神経系)疾患、精神疾患、認知機能障害、炎症性疾患等の分野に焦点を当て、in vivo animal modelおよびin vitro cellular modelを用いた、化合物評価の受託サービスを提供しています。

《サービス概要》 Neurofit社では以下の試験サービスを提供しています。
 
  • Animal / behavioural tests
  • Cellular tests
  • Electromyography (EMG)
  • Histomorphometry
    • 対象疾患の例はこちら

    ・Animal / behavioural tests (動物行動試験) 
     
    • CNSやPNS障害を生じたマウス等における、薬剤や治療による変化を観察します。
      • Cognition
      • Anxiety / Depression
      • Pain / Inflammation
      • Sensori-Motor Function
      • Side Effects
      アッセイの一例

    T-maze    		 Percentage of alternation of mice in the T-maze (Scopolamine model)
       
    Reversal effect of Amphetamine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.   Reversal effect of Caffeine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.

    Passive Avoidance    		 Latency time to enter the black box (Amyloid-β model)
         
    Improved learning/memory in Amyloid-β rats treated with Memantine.
    Acute and chronic Memantine revert the performance of Aβ rats to the level of Sham specimen.     		  Improved learning/memory in Amyloid-β rats treated with Donepezil.
    Chronic Donepezil revert the performance of Aβ rats to the level of Sham specimen.

    Novel Object Recognition    		 Time spent exploring the novel object (Scopolamine model)
      Reversal of Scopolamine-induced cognitive deficit in the novel object recognition assay by Nicotine.
    Scopolamine and/or Nicotine are given at the beginning of the acquisition trial. Retention trial is performed 30 min after the acquisition trial.
    ・Cellular tests (セルベースアッセイ)
     
    • 化合物の神経毒性、神経保護または神経栄養性活性を評価するための多くの細胞モデルを取り揃えています。
      • Rat primary neurons or iPSC neurons cultures
      • Viability and survival
      • Measure of the neurotrophic effect
      • Western-Blot
      • Nerve-Muscle co-culture
      • Inflammation mediator evaluation
      アッセイの一例
    Neurite outgrowth (Procognitive model)
    Effect of reference compounds on neurite outgrowth in cortical neurons culture.
    Donepezil (Aricept®) is a centrally active small molecule used in the treatment of Alzheimer’s disease and different cognitive disorders.
    ・Electromyography (EMG)
     
    • 神経系に影響する様々な疾患で見られる運動感覚の欠損に対して、化合物の影響を評価します。
      • Nerve Function: SNCV (Sensory nerve conduction velocity)
      • Neuromuscular Function: CMAP (Compound muscle action potential)
      アッセイの一例
    CMAP time course (Sciatic Nerve Crush injury in mice)
      Time course of change in the amplitude of CMAP folowing Sciatic Nerve Crush
      Model of nerve / axonal regeneration
    ・Histomorphometry
     
    • 顕微鏡画像の解析により、皮膚や神経等の形成・変化・構造を観察します。
      • IENF (Intra-epidermal nerve fibers)
      • Myelin Thickness: g-ratio
      • Axon Caliber / Distribution
      アッセイの一例
    CMAP time course (Sciatic Nerve Crush injury in mice)
      Comparative photomicrograph of tibial nerve sections showing the status of nerve fibers in Sham operated and sciatic nerve crush specimens at 4 weeks post-injury.
      Nerve morphometry
    (g-ratio: myelin thickness)
    4 weeks after the lesion, axons with diameter ≥ 2.5 µm in lesioned nerve show thinner myelin sheath* than those in sham nerve specimen.
    《対象疾患の例》
      • ADHD
        (Scopolamine)
      • Allergic Contact Dermatitis
        (Ovalbumin, Oxazolone)
      • Alzheimer’s Disease
        (Scopolamine, Aged mouse, Aβ, Methyllycaconitine, LPS-induced inflammation, cortical neurons, iPSC-derived neurons)
      • Amyotrophic Lateral Sclerosis (ALS)
        (neuromuscular junction and nerve fiber morphometry, Glutamate and NMDA, Nerve/Muscle coculture, Spinal motor neurons)
      • Anxiety
      • Depression
        (neurite outgrowth)
      • Epilepsy
        (Pentylenetetrazol)
      • Inflammation (acute)
        (Carrageenan mouse Air Pouch, LPS-induced inflammation, Neuro-inflammation)
      • Multiple Sclerosis
        (RR-EAE, MBP-EAE, Oligodendrocyte Precursor Proliferation)
      • Neuropathic Pain
        (Streptozotocin (STZ), Formalin, Acute pain (Tail Flick, Hot Plate, Paw Pressure))
      • Parkinson’s Disease
        (6-OHDA (hemiparkinson), Haldol (catalepsy), MPP+/6-OHDA)
      • Peripheral Neuropathies
        (Sciatic Nerve Crush, Streptozotocin (STZ), Chemotherapy: IENF)
      • Rheumatoid Arthritis
        (Pristane, Collagen)
      • Schizophrenia
        (MK-801, Phencyclidine, D-amphetamine)
      • Side Effects

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